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one step primescript mirna cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa one step primescript mirna cdna synthesis kit
    One Step Primescript Mirna Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+step+primescript+mirna+cdna+synthesis+kit/One+Step+PrimeScript+RT-PCR+Kit/pmc12595988-73-14-22
    Average 97 stars, based on 2965 article reviews
    one step primescript mirna cdna synthesis kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Upregulated miR-1298-5p sparks inflammatory onset and orchestrates pediatric dry eye disease progression.
    Article Snippet: Purpose Dry eye disease (DED) is a multifactorial chronic ocular surface disorder with increasing annual prevalence in the pediatric population.. MicroRNAs serve as biomarkers, yet the mechanistic role of miR-1298-5p in pediatric DED remains unclear.. This study aims to investigate the role of miR1298-5p in PDED and provide new insights into its clinical treatment.

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain.
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (BioRad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Article Title: The levels of plasma MiR-9 and MiR-106a are associated with the development of peritoneal carcinomatosis in patients with gastric cancer
    Article Snippet: The relative levels of miRNA transcripts were quantified using qRT-PCR with specific primers for 11 metastasis-related miRNAs: miR-10b, miR-9, miR-106a, miR-17, miR-21-5p, miR-200c, miR-516a-3p, miR-34a, miR-139-3p, miR-31, and let-7a (Takara, Japan). .. RNA samples (3 μL each) were polyadenylated and reverse-transcribed into cDNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (Takara). .. PCR amplification was performed on a Bio-Rad IQ5 system using diluted cDNA as a template, SYBR Green, and miRNA-specific primers, according to the manufacturer’s protocol.

    Article Title: Identification and characterization of miRNAs and targets in two poplar varieties with different resistance in responses to Anoplophora glabripennis (Coleoptera) infestation.
    Article Snippet: MicroRNAs (miRNAs) play a crucial role in plant defense against biotic stress, yet their regulatory mechanisms in poplar resistance to Anoplophora glabripennis (ALB) remain poorly understood.. This study investigated the miRNA expression of two distinct poplar varieties, namely Populus tomentosa (resistant) and Populus × euramericana ’74/ 76’ (poplar 107, susceptible), after ALB infestation.. Small RNA sequencing analysis identified 401 miRNAs in both P. tomentosa and poplar 107.

    Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
    Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (Bio-Rad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    cDNA Synthesis:

    Article Title: Upregulated miR-1298-5p sparks inflammatory onset and orchestrates pediatric dry eye disease progression.
    Article Snippet: Purpose Dry eye disease (DED) is a multifactorial chronic ocular surface disorder with increasing annual prevalence in the pediatric population.. MicroRNAs serve as biomarkers, yet the mechanistic role of miR-1298-5p in pediatric DED remains unclear.. This study aims to investigate the role of miR1298-5p in PDED and provide new insights into its clinical treatment.

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain.
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (BioRad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Article Title: The levels of plasma MiR-9 and MiR-106a are associated with the development of peritoneal carcinomatosis in patients with gastric cancer
    Article Snippet: The relative levels of miRNA transcripts were quantified using qRT-PCR with specific primers for 11 metastasis-related miRNAs: miR-10b, miR-9, miR-106a, miR-17, miR-21-5p, miR-200c, miR-516a-3p, miR-34a, miR-139-3p, miR-31, and let-7a (Takara, Japan). .. RNA samples (3 μL each) were polyadenylated and reverse-transcribed into cDNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (Takara). .. PCR amplification was performed on a Bio-Rad IQ5 system using diluted cDNA as a template, SYBR Green, and miRNA-specific primers, according to the manufacturer’s protocol.

    Article Title: Identification and characterization of miRNAs and targets in two poplar varieties with different resistance in responses to Anoplophora glabripennis (Coleoptera) infestation.
    Article Snippet: MicroRNAs (miRNAs) play a crucial role in plant defense against biotic stress, yet their regulatory mechanisms in poplar resistance to Anoplophora glabripennis (ALB) remain poorly understood.. This study investigated the miRNA expression of two distinct poplar varieties, namely Populus tomentosa (resistant) and Populus × euramericana ’74/ 76’ (poplar 107, susceptible), after ALB infestation.. Small RNA sequencing analysis identified 401 miRNAs in both P. tomentosa and poplar 107.

    Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
    Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

    Article Title: High PEEP Activates ITGB1, Inducing Diaphragm Fibrosis During Prolonged Mechanical Ventilation
    Article Snippet: .. For miRNA analysis, total RNA was extracted using the mRNA assay protocol, and cDNA was synthesized from 1000 ng of the total RNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (TaKaRa, Dalian, China). .. The cDNA was diluted with ddH2O and used for reverse transcriptase polymerase chain reaction, as described in TB Green Premix Ex Taq (TaKaRa).

    Article Title: The levels of plasma MiR-9 and MiR-106a are associated with the development of peritoneal carcinomatosis in patients with gastric cancer.
    Article Snippet: The relative levels of miRNA transcripts were quantified using qRT-PCR with specific primers for 11 metastasis-related miRNAs: miR-10b, miR-9, miR-106a, miR-17, miR-21-5p, miR-200c, miR-516a-3p, miR-34a, miR-139-3p, miR-31, and let-7a (Takara, Japan). .. RNA samples (3 μL each) were polyadenylated and reversetranscribed into cDNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (Takara). .. PCR amplification was performed on a Bio-Rad IQ5 system using diluted cDNA as a template, SYBR Green, and miRNA-specific primers, according to the manufacturer’s protocol.

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (Bio-Rad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Amplification:

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain.
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (BioRad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
    Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (Bio-Rad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Quantitative RT-PCR:

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain.
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (BioRad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Article Title: MiR-298 suppresses astrocytic NF-κB activity and neuroinflammation via targeting MyD88 in bone cancer pain
    Article Snippet: Total RNA was retrieved from the spinal cords utilizing TRIzol reagent (Invitrogen). .. Reverse transcription was carried out utilizing a One-step PrimeScript miRNA cDNA Synthesis Kit (Takara), followed by fragment amplification via RT-qPCR using iQSYBR Green mix (Bio-Rad) and a target-specific primer set. .. The reactions were executed utilizing a PCR platform (Applied Biosystems) that could also calculate measurements.

    Purification:

    Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
    Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

    Isolation:

    Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
    Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

    Synthesized:

    Article Title: High PEEP Activates ITGB1, Inducing Diaphragm Fibrosis During Prolonged Mechanical Ventilation
    Article Snippet: .. For miRNA analysis, total RNA was extracted using the mRNA assay protocol, and cDNA was synthesized from 1000 ng of the total RNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (TaKaRa, Dalian, China). .. The cDNA was diluted with ddH2O and used for reverse transcriptase polymerase chain reaction, as described in TB Green Premix Ex Taq (TaKaRa).



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